Purification and characterisation of haemagglutinin from Bordetella bronchiseptica
- 1 November 1993
- journal article
- Published by Microbiology Society in Journal of Medical Microbiology
- Vol. 39 (5) , 388-392
- https://doi.org/10.1099/00222615-39-5-388
Abstract
A surface protein of Bordetella bronchiseptica was purified in one step by affinity chromatography with bovine submaxillary mucin coupled to agarose. The purified protein, with a mol. wt of 200 kDa and an iso-electric point of pI 6.5, showed haemagglutinating activity for bovine erythrocytes. This haemagglutinin (HA) inhibited the adherence of B. bronchiseptica to a rat lung cell line (L2) and was able to bind to N-acetylneuraminic acid. These findings suggest that the HA of B. bronchiseptica is an adhesin.Keywords
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