Clinicopathological study of proliferating cell nuclear antigen (PCNA) of hepatocytes in primary biliary cirrhosis
- 1 January 1994
- journal article
- research article
- Published by Springer Nature in The Esophagus
- Vol. 29 (1) , 56-60
- https://doi.org/10.1007/bf01229074
Abstract
The DNA synthesis activities of hepatocytes in primary biliary cirrhosis (PBC) and other chronic liver diseases and control subjects were examined by staining proliferating cell nuclear antigen (PCNA) with anti-PCNA monoclonal antibody. The number of PCNA-positive cells (PCNA value) was significantly higher in PBC (375±281 parts per thousand; ppt) than in other chronic liver diseases, i.e., chronic hepatitis (95±83 ppt), liver cirrhosis (72±71 ppt), and alcoholic liver disease (73±56 ppt), and in control subjects (11±14 ppt). The PCNA value of PBC in stages I-III of Scheuer's classification was remarkably high, while in stage IV it was low. Even in identical, Scheuer's stages, the PCNA value of PBC was higher in patients who were not given ursodeoxycholic acid (UDCA) than in those who received UDCA. In identical patients, the PCNA value was lowered significantly after UDCA treatment. It was concluded that the DNA synthesis activity of PBC in stages I-III was accelerated and that UDCA can alleviate the abnormality in DNA synthesis activity.Keywords
This publication has 28 references indexed in Scilit:
- Detection of proliferating liver cells in various diseases by a monoclonal antibody against DNA polymerase-α: With special reference to the relationship between hepatocytes and sinusoidal cellsHepatology, 1991
- Ursodeoxycholic acid (UDCA) in the treatment of chronic cholestatic diseasesBiochimie, 1991
- Changes in cell nuclei during S Phase: Progressive chromatin condensation and altered expression of the proliferation-associated nuclear proteins Ki-67, cyclin (PCNA), p105, and p34*1Experimental Cell Research, 1991
- Laparoscopic Findings of Asymptomatic Primary Biliary Cirrhosis —Particular investigation in early PBC—Digestive Endoscopy, 1991
- Clinical and clinico-pathological studies on early primary biliary cirrhosis.Kanzo, 1990
- Pathologic study of primary biliary cirrhosis of early histologic stages presenting cholestatic jaundiceLiver International, 1988
- S-Phase cells in diseased human liver determined by an in Vitro BrdU-anti-BrdU methodHepatology, 1988
- IS URSODEOXYCHOLIC ACID AN EFFECTIVE TREATMENT FOR PRIMARY BILIARY CIRRHOSIS?The Lancet, 1987
- Characterization of proliferating cell nuclear antigen recognized by autoantibodies in lupus sera.The Journal of Experimental Medicine, 1984
- Monoclonal Antibody to 5-Bromo- and 5-Iododeoxyuridine: A New Reagent for Detection of DNA ReplicationScience, 1982