Induction of Sister Chromatid Exchanges in Chinese Hamster Cells by Antitumor Agents and Its Relation to Chromosome Aberrations

Open Access

1 January 1978

journal article
research article
Published by Japan Society for Cell Biology in Cell Structure and Function

Vol. 3 (4) , 341-347
https://doi.org/10.1247/csf.3.341

Abstract

Various antitumor agents were used to investigate the induction of sister chromatid exchanges (SCEs), one of the most sensitive tests for detecting the effects of mutagenic carcinogens. Antimetabolites in nucleic acid synthesis and inhibitors of protein synthesis were tested on Chinese hamster cells in vitro. These agents increased the frequency of SCEs even after a one hour exposure, and during the limited stage of the last cell division cycle beforefixation. In chase experiments, the response curves of SCEs and chromosomeaberrations were somewhat similar, but seemed to fluctuate independent of each other. Chromosome breaks and gaps induced by the agents were not necessarily associated with SCEs at the breakpoints. Possible relationships between SCE formation and chromosome aberrations are discussed.

This publication has 1 reference indexed in Scilit:

Sister chromatid exchange as an assay for genetic damage induced by mutagen-carcinogens. I. In vivo test for compounds requiring metabolic activation
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1976