Decrease in apparent Km for oxygen after stimulation of respiration of rat polymorphonuclear leukocytes
Open Access
- 5 September 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 161 (1) , 60-64
- https://doi.org/10.1016/0014-5793(83)80730-3
Abstract
The respiratory burst of polymorphonuclear leukocytes, induced by the addition of chemotactic peptide (N‐formyl‐methionyl—leucyl—phenylalamine) and cytochalasin B was found to consist of two phases. The first phase of very rapid oxygen uptake lasted 1–3 min. and was followed by a second more prolonged phase of lower magnitude. The apparent K m for oxygen of unstimulated cells was 9.6 ± 0.67 μM, while that of the second phase of stimulation was 3.7 ± 1.6 μM oxygen. The possibility that lowered oxygen concentrations may regulate polymorphonuclear leukocyte activity in some pathological conditions is discussed.Keywords
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