Phosphate Translocator of Mesophyll and Bundle Sheath Chloroplasts of a C4 Plant, Panicum miliaceum L.
- 1 December 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 91 (4) , 1507-1511
- https://doi.org/10.1104/pp.91.4.1507
Abstract
The phosphate translocator was identified in the envelope membranes of both mesophyll and bundle sheath chloroplasts of Panicum miliaceum L. by labeling with [1,2-3H]1,2-(2,2''-disulfo-4,4''-diisothiocyano)diphenylethane ([3H]H2DIDS) and by using SDS-PAGE. Assay of 32Pi uptake by the chloroplasts showed that the phosphate translocators of both types of chloroplasts have a higher affinity for phosphoenolpyruvate than the C3 counterpart and can be regarded as C4 types.Keywords
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