The inactivation profile of rabbit muscle creatine phosphokinase in biological fluids.

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Abstract
The inactivation profiles of rabbit muscle creatine phosphokinase (CPK) in heparinized whole blood and plasma under various pH conditions at 39.degree. C were investigated. Under pH 7.40, at 39.degree. C, the inactivation profile of CPK in heparinized whole blood follows apparent first-order kinetics. The inactivation rate constant is 0.054 h-1 and is very close to that observed in patients with acute myocardial infarction, but much less than that resulting from exercise. The nature of the inactivation of CPK in vitro is presumably thermal denaturation, and there is no evidence to show that blood cells or dissolved O2 are involved. The inactivation profiles of CPK in heparinized whole blood at pH other than 7.40 appear to be biphasic. The regression equations and the half-lives are presented.