Effect of Dithiothreitol on Yeast: Sphaeroplast Formation and Invertase Release

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Abstract
Summary: The rate of sphaeroplast formation from yeast was greater with dithiothreitol (DTT) in the reaction mixture than with 2-mercaptoethanol (2-ME) and the sphaeroplast population so produced was more rapidly and completely lysed in a non-isotonic environment. Incubation with DTT, but not 2-ME, allowed rapid formation of sphaeroplasts from yeast in stationary phase. DTT and 2-ME readily released invertase from Saccharomyces carlsbergensis, but only DTT released invertase from S. cerevisiae. DTT-induced release of invertase by both yeasts was dependent on DTT concentration, pH, temperature, metal ions and yeast age, but these yeasts responded differently to oxidation and to release under suboptimal conditions.

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