Biochemical Typing of Urinary Escherichia Coli Strains by Means of the Api 20 E Enterobacteriaceae System

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Abstract
With the API 20 E Enterobacteriaceae system of biochemical testing, a biotype, coded numerically, was determined for each of 574 strains of E. coli isolated from [human] patients with urinary tract infection. The serotypes of the strains were also determined. Fifty-five different biotypes were identified, 2 accounting together for 42% of the strains examined and 7 others each accounting for between 8.4 and 1.9%. There was little correlation between biotype and serotype. Fifty pairs of strains were isolated from patients before [antibiotic] treatment. In 43 the biotype and serotype of both strains of each pair were the same. In 6 pairs the biotypes but not the serotypes differed, the difference being limited to the results of the tests for lysine decarboxylase. The biotypes of the strains of the remaining pair differed widely although their serotypes were the same. This method of biotyping offers a simple but accurate way of discriminating between recrudescent urinary tract infection caused by E. coli and that due to reinfection.

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