Basement membrane collagens. Cyanogen bromide peptides of the D chain from porcine kidney

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Abstract
An .alpha. chain size collagenous component, designated as the D chain, was isolated from the pepsin digest of porcine kidney cortices. Reduction of the D chain with 2-mercaptoethanol and carboxymethylation with iodoacetic acid resulted in the formation of two smaller components of 75,000 and 15,000 MW. The 75,000 and 15,000 MW components were separated by molecular sieve chromatography on a column of agarose A-5M. The 75,000 MW component was cleaved with CNBr in 70% formic acid. The resulting CNBr peptides were isolated by a combination of ion exchange and molecular sieve chromatography and were characterized for amino acid contents and MW. A total of 7 CNBr peptides were obtained, which together accounted for the amino acid content of the intact 75,000 MW component.