Detection of Complement-Dependent Antibody to Tumor Cells in Sera of Strain-2 Guinea Pigs Cured of Their Tumors by BCG Treatment
- 1 March 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 56 (3) , 623-626
- https://doi.org/10.1093/jnci/56.3.623
Abstract
Sera of strain-2 guinea pigs (cured of line-10 tumor by BCG therapy) were tested for complement-dependent, cytotoxic antibody. About 30% of the sera tested contained significant cytotoxic activity with the addition of human, but not syngeneic, complement. Using papain pretreated line-lO cells, we detected antibody in about 50% of the sera with syngeneic sera as the source of complement. Antibody to line-10 was also demonstrated in selected sera by indirect fluore,scence and the C1 fixation and transfer test.Keywords
This publication has 1 reference indexed in Scilit:
- Demonstration of Cellular Antigens on Sarcoma Cells by an Indirect125 I-Labeled Antibody Technique2JNCI Journal of the National Cancer Institute, 1968