Stimulation and Inhibition of Synthesis of Ovulating Hormone (OH) in Organ Culture by Subcellular Fractions of Rat Medial Basal Hypothalami

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Abstract
Subcellular fractions of rat medial basal hypothalami were examined for their ability to stimulate the synthesis of ovulating hormone (OH) in a pituitary organ culture system. The hypothalamic stimulator(s) of OH synthesis (HOHS) was associated with the 1–15,000 × g participates and was not retained by a 0.5 μ (diameter pore size) Nuclepore filter. This fraction appeared to contain many of the electron dense vesicles present in this region of the hypothalamus. Sepharose 2B gel filtration of these participates revealed not 1 but 2 zones of HOHS activity separated by a clear, distinct area containing an inhibitor of OH synthesis. Similar data were obtained using the Bogdanove- Gay ovarian ascorbic acid depletion assay for LH in place of the ovulating hormone assay. That the hypothalamus may, in addition to producing gonadotropin releasing factors, be producing gonadotropin inhibitory substances is discussed. Such inhibitory substances provide a means whereby gonadotropin production could be suppressed. (Endocrinology88: 969, 1971)

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