Chlorpromazine Inhibition of p‐Aminophenol Glucuronidation by Rat Hepatoma Cells in Culture

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Abstract
Chlorpromazine (0.01 mM to 0.15 mM) inhibits the glucuronidation of p‐aminophenol and p‐nitrophenol in a clonal strain of rat hepatoma cells, MH1C1, grown in culture, producing 50% inhibition of p‐aminophenol glucuronidation in a serumfree incubation medium at 0.07 mM. Chlorpromazine, at concentrations of 0.05 mM to 1.0 mM, also inhibits the glucuronidation of p‐aminophenol in a homogenate prepared from the rat hepatoma cells (50% inhibition at 0.25 mM); this inhibition was found to be non‐competitive. The effect of chlorpromazine is enhanced when serum is excluded from the incubations.