A Source of Error in Tracer Experiments with P32

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Abstract
A solution of P32 containing about lxl0-4 mg./ml. of total phosphorus was drawn once into 2 new 2 ml. pipettes and into 2 new ml. pipettes that had been coated with silicone; the pipettes were drained and then washed with 3 washings of 2 ml. of distilled water each. The washings were evaporated in sample cups, and the number of radioactive disintegrations in the residue determined. Considerable activity was found in the washings[long dash]6000 cpm/ml. in the 1st. wash of the un-coated pipette. The counts were much lower in the washes from the coated pipette, and there was no activity in the 3d wash from coated pipettes. Use of high levels; (1 mg./ml.) of carrier P at pH 4.5 minimizes adsorption of P32 on glassware; and, when low carrier P concn. or high pH are required, glassware should be coated with silicone. The method of coating is described.

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