PHOTOBIOLOGICAL STUDIES ON ESCHERICHIA COLI AT LOW TEMPERATURES

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Abstract
Inactivation of E. coli strain B, by u.-v. radiation takes place also at subfreezing temps. At pH 8 to 10 the reactivation rate is accelerated with increasing alkalinity only in the frozen state. The spectral region between 300 to 400 m[mu] can produce a partial, but irreversible inactivation in the frozen state. Bacteria inactivated by u.-v. either in the frozen or liquid phase can be photoreactivated only in the liquid phase. This suggests that the inactivation process represents the only true photochemical reaction. Bacteria inactivated and stored in frozen state, can be reactivated after several days.