The RPL12 Lymphomatosis Virus in Chicken Embryo and in Chicken-Embryo Tissue Culture. Morphological and Cytochemical Observations

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Abstract
The alterations induced by the RPL12 lymphomatosis virus in chicken-embryo liver tissue culture and in chicken embryo were described. In the tissue-culture system, large intranuclear inclusions leading to eventual cell destruction were observed; the number of inclusions and the time necessary for their formation were related to the amount of virus introduced. Cytochemical studies indicated that the inclusions were rich in deoxyribonucleic acid. At the terminal stage the inclusions consisted of a dense central body surrounded by a chromophobe halo and an external ring of basophilic material. The evolution and morphology of these inclusions seemed to be fairly characteristic for this virus. In the 12-day-old chicken embryo, undiluted virus preparations regularly induced a series of changes: Period I (0 to 48 hours)—Intranuclear inclusions formed in the hepatic cells, with morphological and cytochemical characteristics similar to those observed in tissue culture. Period II (48 to 72 hours)—Large basophilic cells proliferated in the mesenchyma of the liver and other organs. Cells with inclusions were still present. On the basis of its characteristics, the proliferative process has been considered similar to the initial phase of the experimental lymphomatosis in the adult bird. Period III (72 hours to hatching time)—Proliferative lesions persisted in the liver and were accompanied by large areas of necrosis. The relationship of the virus to the characteristic cytolytic and proliferative changes was discussed and some evidence was brought forward for an interpretation of the two processes as an expression of the same virus.

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