Production of Synnemata on Defined Agar Media and Its Relation to Pathogenicity of Ceratocystis ulmi

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Abstract
Defined agar media were developed which were suitable for synnema production by some isolates of C. ulmi [the Dutch elm disease fungus]. Several media supported synnema production, but the best medium contained (per liter): 2.0 g glucose, 0.08 g L-asparagine, macro- and microelements, pyridoxine and 0.50 g linoleic acid. Production of synnemata on this medium varied among isolates of C. ulmi with known pathogenicities, but more aggressive isolates generally produced more synnemata than less aggressive ones.

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