Infection of Protoplasts from Several Brassica Species with Cauliflower Mosaic Virus Following Inoculation Using Polyethylene Glycol

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Abstract
Using a polyethylene glycol-mediated uptake procedure, protoplasts from a range of Cruciferous spp. were infected with cauliflower mosaic virus; they include Moricandia arvensis, a plant not known to be susceptible. With this technique %G 90% of turnip protoplasts were infected, each producing an average of 104-105 viral genome equivalents. Filter hybridization and fluorescent antibody staining showed that virus synthesis in turnip protoplasts reaches a maximum 65-96 h postinoculation. In protoplasts from some other species the time course was different; viral DNA synthesis in rape reached a maximum 24-48 h post-inoculation, .apprx. 48 h before the maximum accumulation of viral antigen.