PREPARATION AND CHEMICAL CHARACTERISTICS OF ENDOTOXIC LIPOPOLYSACCHARIDE FROM THREE STRAINS OF SPHAEROPHORUS NECROPHORUS

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Abstract
Lipopolysaccharide (LPS) has been isolated from the supernatant fluid after ultracentri‐fugation of the water phase following phenol‐water extraction of Sphaerophorus necrophorus, strains ATCC 12290, 3328 Institut Pasteur and N167 Fievez. The purification procedure included digestion of the supernatant fluid with ribonuclease and deoxyribonuclease, gel filtration on Bio‐Gel A—1.5 m and ion exchange chromatography on DEAE‐cellulose. LPS from strain N167 contained small amounts of 2‐keto‐3‐deoxy‐octonate, and the major sugar components were heptose, galactose, glucose and glucosamine. 2‐keto‐3‐deoxy‐octonate and heptose were not present in LPS from strains 12290 and 3328. The sugar components identified were galactose, ' glucose, mannose, fucose, rhamnose, glucosamine and galacto‐samine. The endotoxic potency of the preparations was low.