Evaluation of an Enzyme-linked Immunosorbent Assay Method for Predicting Brown Patch Infection in Turf

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Abstract
A field investigation was conducted during 1991 and 1992 to determine the effectiveness of enzyme-linked immunosorbent assay (ELISA) to predict brown patch (Rhizoctonia solani Kühn) infection events in `Caravelle' perennial ryegrass (Lolium perenne L.). Turfgrass samples were collected either between 7:00 and 8:00 am or 4:00 and 5:00 pm, and from plots mowed to a height of either 1.7 or 4.5 cm. Pathogen detection levels were generally higher in am-sampled turf and in plots mowed to a height of 4.5 cm. During 2 years, only 7 of 15 infection events were predicted from samples collected from high-cut turf and only three from samples collected from low-cut turf. While this technology is useful for confirming the presence of R. solani, it was unreliable for predicting infection events.

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