cAMP directly facilitates Ca‐induced exocytosis in bovine lactotrophs
- 29 October 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 273 (1-2) , 150-154
- https://doi.org/10.1016/0014-5793(90)81072-v
Abstract
We have used the whole cell patch clamp technique on single prolactin-secreting bovine lactotrophs to measure plasma membrane capacitance (C m), an index of membrane surface area, under voltage-clamp during cytosol dialysis with Ca and cAMP. cAMP increased the magnitude and rate of Ca-induced exocytosis (C m increase) without affecting membrane conductance; however, cAMP had no detectable effect on C m when intracellular Ca was low. We thus report new evidence that cAMP can facilitate Ca-induced secretion in a synergistic fashion, by acting directly on the secretory apparatus, independently of membrane conductance activation.Keywords
This publication has 31 references indexed in Scilit:
- Dual effects of G‐protein activation on Ca‐dependent exocytosis in bovine lactotrophsFEBS Letters, 1989
- A role for calpactin in calcium-dependent exocytosis in adrenal chromaffin cellsNature, 1989
- Inhibition of exocytosis by intracellularly applied antibodies against a chromaffin granule-binding proteinNature, 1989
- A cAMP-Regulated Chloride Channel in Lymphocytes That Is Affected in Cystic FibrosisScience, 1989
- Overcoming barriers to exocytosisTrends in Neurosciences, 1987
- Currents through the fusion pore that forms during exocytosis of a secretory vesicleNature, 1987
- Synapsin I bundles F-actin in a phosphorylation-dependent mannerNature, 1987
- Kinetic analysis of the triggered exocytosis/endocytosis secretory cycle in cultured bovine adrenal medullary cells.The Journal of cell biology, 1986
- Dopamine-Induced Physical Barrier to Inhibit Prolactin Release in Mammotrophs Disappears during Resumption of Prolactin SecretionNeuroendocrinology, 1985
- Endogenous phosphorylation and dephosphorylation of microtubule‐associated proteins isolated from bovine anterior pituitaryFEBS Letters, 1975