Prediction of peptides observable by mass spectrometry applied at the experimental set level
Open Access
- 1 November 2007
- journal article
- Published by Springer Nature in BMC Bioinformatics
- Vol. 8 (S7) , S23
- https://doi.org/10.1186/1471-2105-8-s7-s23
Abstract
When proteins are subjected to proteolytic digestion and analyzed by mass spectrometry using a method such as 2D LC MS/MS, only a portion of the proteotypic peptides associated with each protein will be observed. The ability to predict which peptides can and cannot potentially be observed for a particular experimental dataset has several important applications in proteomics research including calculation of peptide coverage in terms of potentially detectable peptides, systems biology analysis of data sets, and protein quantification.Keywords
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