Purification and characterization of a β-glucosidase from Streptomyces lividans 66
- 1 January 1990
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 36 (1) , 53-56
- https://doi.org/10.1139/m90-009
Abstract
An intracellular β-1, 4-D-glucosidase (EC 3.2.1.21) was isolated from the mutant strain HP-3 of Streptomyces lividans 66 which produced about 12 times more enzyme than the wild-type strain. The purification was carried out by anion exchange column chromatography followed by high-performance liquid chromatography on DEAE and on molecular sieve columns. The enzyme is glycosylated and has an apparent Mr of 51 000 and a pI of 4.3. Its activity was optimal at pH 6.5 and at a temperature of 40 °C. The Km and the Vmax on cellobiose were 3.1 mM and 65.6 μmol min−1 mg−1 of enzyme. Key words: β-glucosidase, Streptomyces lividans, purification, characterization.This publication has 8 references indexed in Scilit:
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