Characterization of Saccharomyces cerevisiae deficient in expression of phospholipase D
- 15 February 1996
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 314 (1) , 15-19
- https://doi.org/10.1042/bj3140015
Abstract
A gene encoding phospholipase D (PLD) in Saccharomyces cerevisiae was identified. The 195 kDa product of PLD1 has 24% overall sequence identity with a plant PLD. Expression of yeast PLD activity was eliminated by one-step gene disruption. Yeast haploids lacking PLD activity were deficient in growth on non-fermentable carbon sources. Diploids lacking expression of PLD1 were unable to sporulate.Keywords
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