DRB-induced premature termination of late adenovirus transcription
- 1 April 1978
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 272 (5654) , 590-593
- https://doi.org/10.1038/272590a0
Abstract
Exposure of [human cervical carcinoma] HeLa cells to 5,6-dichloro-1-.beta.-D-ribofuranosylbenzimidazde (DRB) late in the course of adenovirus 2 infection results in the inhibition of virus-specific RNA synthesis from all parts of the previously identified, very long (.apprx. 28 kilobases) late transcriptional unit, except for the first .apprx. 400-800 nucleotides. DRB probably acts not as an inhibitor of RNA chain initiation, but causes premature chain termination close to the promoter. A practical aspect of these findings may be that RNA sequences near promoter sites that are responsible for mRNA formation can be isolated from DRB-treated cells.This publication has 23 references indexed in Scilit:
- Halobenzimidazole Ribosides and RNA Synthesis of Cells and VirusesPublished by Elsevier ,1978
- The initiation sites for RNA transcription in Ad2 DNACell, 1977
- The definition of a large viral transcription unit late in Ad2 infection of HeLa cells: Mapping of nascent RNA molecular labeled in isolated nucleiCell, 1977
- The definition of a large viral transcription unit late in Ad2 infection of HeLa cells: Mapping by effects of ultraviolet irradiationCell, 1977
- The topography and transcription of the adenovirus genomeCell, 1977
- 5,6-Dichloro-1-β-D-Ribofuranosylbenzimidazole Inhibits Initiation of Nuclear Heterogeneous RNA Chains in HeLa CellsScience, 1976
- Initiation inhibition and reinitiation of the synthesis of heterogeneous nuclear RNA in living cellsNature, 1976
- Adenovirus-2 mRNA is transcribed as part of a high-molecular-weight precursor RNA.Proceedings of the National Academy of Sciences, 1975
- Inhibition of Balbiani ring RNA synthesis at the initiation level.Proceedings of the National Academy of Sciences, 1975
- Transcription of the tryptophan operon in Escherichia coli: Rifampicin as an inhibitor of initiationJournal of Molecular Biology, 1970