Effect of Interleukin 10 on the Hematopoietic Progenitor Cells from Patients with Aplastic Anemia

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Abstract
The overproduction of cytokines with inhibitory effects on hematopoiesis is considered to play a role in the pathogenesis of aplastic anemia. While interleukin 10 (IL‐10) is a cytokine production inhibitory factor, the possibility of immunosuppressive therapy using IL‐10 for aplastic anemia has not been explored. In this study, therefore, we examined the effect of IL‐10 on progenitor cells obtained from seven patients with severe aplastic anemia. Our study indicated that IL‐10 dramatically enhanced the erythroid colony formation in a dose‐dependent manner in two of the seven cases examined. When we examined the concentration of cytokines in the culture supernatants of unstimulated bone marrow cells, the spontaneous production of interferon‐γ (IFN‐γ) was observed in one of these two cases, and this production was completely inhibited by addition of IL‐10. These findings suggested that IL‐10 enhanced the erythroid colony formation by inhibiting the pathological production of IFN‐γ in this case. This study provides an experimental support for the clinical application of IL‐10 in some patients with aplastic anemia.