Analysis of 5-chloro-7-iodo-8-quinolinol conjugates by high performance liquid chromatography.
Open Access
- 1 January 1975
- journal article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 23 (9) , 2173-2174
- https://doi.org/10.1248/cpb.23.2173
Abstract
An analytical method of 5-chloro-7-iodo-8-quinolinol (Clioquinol or Chinoform) (CF) conjugates was established by high performance liquid chromatography. The chromatographic conditions were as follows : column, SAX (50 cm×2 mm i.d.) ; mobile phase, I (0.02M borate buffer-0.1M KCl, pH9.5), II (0.02M borate buffer-0.5M NaClO4, pH 9.5) ; gradient, 0→25% (II/I+II) at 3%/min ; column pressure, 500 psi ; flow rate, 1 ml/min ; detector, ultraviolet-detector at 254 mμ. Urine samples of a man administered CF were injected directly to the column, and the major metabolite, CF glucuronide was determined, while very small amounts of CF sulfate was detectedKeywords
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