Subcellular Distribution of Carbonic Anhydrase and Na+,K+‐ and HCO3‐‐ATPases in Brains of DBA and C57 Mice

Publisher Website
Google Scholar
Abstract
DBA/2J mice are susceptible to audiogenic seizures (AS) in an age-dependent manner, susceptibility being maximal at 21 days of age and declining thereafter. DBA, as compared with AS-resistant C57BL/6J (C57) mice, had higher carbonic anhydrase (CA) activity in cerebral cortex, brainstem and cerebellum homogenates at 21 days of age. CA activity was also increased in cytosolic (82%), microsomal (167%) and myelin (68%) subcellular fractions from cerebral cortex, and in cytosolic (51%) and mitochondrial (102%) fractions from brainstem of DBA mice at 21 days of age. DBA mice had a higher Na+,K+-ATPase activity in myelin from cerebral cortex, and a lower HCO3--ATPase activity in mitochondria from brainstem. The differences in CA activity in the cerebral cortex and in HCO3--ATPase were not present at 110 days of age, when DBA mice are not longer susceptible to ASs. Because CA and HCO3--ATPase are involved in maintaining a proper ionic environment for neuronal function, alterations in activity of these enzymes may be related to the age-dependent changes in AS susceptibility in DBA mice.