The methylation state of poly A-containing-messenger RNA from cultured hamster cells
- 1 January 1975
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 2 (10) , 1653-1668
- https://doi.org/10.1093/nar/2.10.1653
Abstract
The poly A-containing mRNA of cultured hamster (BHK-21) cells has been examined with regard to methylation status. Steady state-labeled mRNA was obtained by incubating cells for 20-22h in the presence of [methyl-3H]-methionine and 32Pi. The degree of methylation of this RNA was 1.8 methyl groups per 1000 nucleotides, or 4-5 methyl groups on the average per molecule. The nature of the methylated residues was determined by paper chromatography and electrophoresis of acid and alkaline hydrolysates, by DEAE cellulose chromatography of alkaline hydrolysates and of T2 RNase digests, and by examining the effect of subjecting samples to "beta-elimination." Approx. half of the methyl groups occurred in standard ("internal") linkage, 10% as m5Cp and 40% as m6Ap residues. The remainder occurred at least for the most part in "blocked" 5'-termini with the presumptive structure m7G(5')ppp(Nm)p.., where Nm was Gm, m6Am, Um, or Cm.Keywords
This publication has 17 references indexed in Scilit:
- Reovirus messenger RNA contains a methylated, blocked 5'-terminal structure: m-7G(5')ppp(5')G-MpCp-.Proceedings of the National Academy of Sciences, 1975
- The messenger-like properties of the poly(A)+ RNA in mammalian mitochondriaCell, 1974
- Sequences containing methylated nucleotides at the 5′ termini of messenger RNAs: Possible implications for processingPublished by Elsevier ,1974
- Identification of Methylated Nucleosides in Messenger RNA from Novikoff Hepatoma CellsProceedings of the National Academy of Sciences, 1974
- The methylated nucleotide sequences in HeLa cell ribosomal RNA and its precursorsJournal of Molecular Biology, 1974
- Methylated nucleotide content of mitochondrial ribosomal RNA from hamster cellsJournal of Molecular Biology, 1974
- In vitro tnethylation of nascent reovirus mRNA by a virion-associated methyl transferaseNucleic Acids Research, 1974
- Messenger RNA turnover in mouse L cellsJournal of Molecular Biology, 1973
- Mitochondrial RNA from cultured animal cells. Distinctive high-molecular-weight and 4 s speciesJournal of Molecular Biology, 1970
- The occurrence and distribution of thymine and three methylated-adenine bases in ribonucleic acids from several sourcesBiochemical Journal, 1958