Revised structure of a trehalose-containing immunoreactive glycolipid of Mycobacterium tuberculosis
- 1 March 1991
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 78 (2-3) , 171-175
- https://doi.org/10.1016/0378-1097(91)90153-2
Abstract
Nuclear magnetic resonance spectroscopy, fast-atom bombardment mass spectrometry as well as various chemical degradations and chromatographic techniques were used to re-examine the structure of a highly immunoreactive glycolipid previously described in Mycobacterium tuberculosis (strain Canetti) as a 2,3-diacyl trehalose 2'-sulfate (labelled SL-IV). Ion exchange chromatography allowed the recognition of a neutral and an acidic glycolipid, indistinguishable on conventional silica gel. The neutral glycolipid was shown to be serologically identical to SL-IV and its structure was established as 2,3-diacyl trehalose. It corresponded to the non-chemically defined highly observed immunoreactive lipid previously recognized by others in M. tuberculosis (H37Rv).Keywords
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