Ovarian cancer marker of 11.7 kDa detected by proteomics is a serum amyloid A1
- 16 September 2005
- journal article
- research article
- Published by Wiley in Proteomics
- Vol. 5 (14) , 3790-3797
- https://doi.org/10.1002/pmic.200401205
Abstract
In this study, to reduce the number of major plasma components, we examined thermostable plasma fractions to search for a biomarker of ovarian cancer. An apparent cancer biomarker of 11.7 kDa was detected in these fractions using ProteinChip SELDI‐TOF mass spectrometry system. This peak invariably appeared with another close peak of about 11.5 kDa, suggesting that it is a derivative of a larger mass molecule. Of 27 cancer plasma specimens, 15 (55.6%) demonstrated this peak pair, whereas only 2 of 34 controls specimens (5.8%) were shown to express it with low intensity. Using a method involving cysteine modification by 4‐vinylpyridine (4‐VP), 2‐DE and HPLC, these peaks were identified by mass spectrometry as serum amyloid A1 (11.68 kDa) and its N‐terminal arginine‐truncated form (11.52 kDa).Keywords
This publication has 16 references indexed in Scilit:
- Identification of biomarkers for ovarian cancer using strong anion-exchange ProteinChips: Potential use in diagnosis and prognosisProceedings of the National Academy of Sciences, 2003
- Clinical proteomics: Written in bloodNature, 2003
- Identification and validation of a potential lung cancer serum biomarker detected by matrix‐assisted laser desorption/ionization‐time of flight spectra analysisProteomics, 2003
- Protocols for disease classification from mass spectrometry dataProteomics, 2003
- Machine learning approaches to lung cancer prediction from mass spectraProteomics, 2003
- The Human Plasma ProteomeMolecular & Cellular Proteomics, 2002
- Proteomic patterns in serum and identification of ovarian cancerThe Lancet, 2002
- Use of proteomic patterns in serum to identify ovarian cancerPublished by Elsevier ,2002
- Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gelsElectrophoresis, 1987
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976