The quantitative analysis of nanoplankton: a study of methods

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Abstract
The Filter-Transfer-Freeze (FTP) technique has been compared to traditional techniques (Utermöhl, electronic particle counter, counting chamber, etc.) for ability to give quantitative cell-density estimates of either natural water samples or Laboratory cultures. The FTF technique was found to be equal or superior to these other methods for nanoplankton populations. In addition, the FTF technique offers many advantages such as (i) good optical quality of the sample. (ii) flexibility in preservation and fixation of sample, (iii) convenience of multiple optical modes for sample analysis and (iv) rapid and easy preparation allowing accurate analysis of oceanic nanoplankton on board ship.

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