Effect of vitamin B on the growth of fibroblasts

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Abstract
Fibroblasts were obtained from the choroidal and sclerotic layers of the eyes of 9 day chick embryos and cultured by the hanging drop method for at least ten passages before being used for experiments. Extracts of the brains of pigeons suffering from beri beri contained substances which promoted a satisfactory growth of fibroblasts, but the growth was significantly less than that with extracts of brains of normal pigeons. The addition of yeast extract deficient in Vit. B1 and biotin significantly improved the growth of fibroblasts grown in a medium deficient in vit. B. The addition of vit. B1 as aneurin chloride hydrochloride did not increase growth whether the medium was deficient in vit. B content or not. The vit. B complex, or some component of it has a specific effect on growth in vitro, but the growth promoting factor is probably not aneurin.

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