Therapeutic potential of antiidiotypic single chain antibodies with yeast killer toxin activity
- 1 February 1997
- journal article
- Published by Springer Nature in Nature Biotechnology
- Vol. 15 (2) , 155-158
- https://doi.org/10.1038/nbt0297-155
Abstract
Single chain fragment (ScFv) antiidiotypic antibodies (antilds) of a killer toxin (KT) from the yeast Pichia anomala have been produced by recombinant DNA methodology from the splenic lymphocytes of mice immunized by idiotypic vaccination with a KT-neutralizing monoclonal antibody (Mab KT4). ScFv KT-like antilds (KTIdAb) react with specific Candida albicans KT cell wall receptors (KTR) exerting a candidacidal activity in vitro could be neutralized by adsorption with Mab KT4. ScFv KTIdAb displayed an effective therapeutic activity in an experimental model of rat candidal vaginitis.Keywords
This publication has 23 references indexed in Scilit:
- Antibodies, killer toxins and antifungal immunoprotection: a lesson from nature?Immunology Today, 1997
- Immunotoxins against cancerBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1994
- Inhibitory effect of a yeast killer toxin to the in vitro Pneumocystis carinii attachmentSerodiagnosis and Immunotherapy in Infectious Disease, 1993
- Specificity and Protective Activity of Murine Monoclonal Antibodies Directed Against the Capsular Polysaccharide of Type III Group B StreptococciHybridoma, 1992
- By-passing immunizationJournal of Molecular Biology, 1991
- PUTATIVE VIRULENCE FACTORS OF CANDIDA ALBICANSAnnual Review of Microbiology, 1991
- Making antibody fragments using phage display librariesNature, 1991
- Phage antibodies: filamentous phage displaying antibody variable domainsNature, 1990
- Idiotypic Mimicry of Biological ReceptorsAnnual Review of Immunology, 1986
- Anti-Idiotypic Antibodies and DiseaseImmunological Investigations, 1986