Molecular cloning of the Ecotin gene in Escherichia coli
Open Access
- 5 August 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 287 (1-2) , 53-56
- https://doi.org/10.1016/0014-5793(91)80014-t
Abstract
The nucleotide sequence of a 876 bp region in E. coli chromosome that encodes Ecotin was determined. The proposed coding sequence for Ecotin is 486 nucleotides long, which would encode a protein consisting of 162 amino acids with a calculated molecular weight of 18 192 Da. The deduced primary sequence of Ecotin includes a 20‐residue signal sequence, cleavage of which would give rise to a mature protein with a molecular weight of 16 099 Da. Ecotin does not contain any consensus reactive site sequences of known serine protease inhibitor families, suggesting that Ecotin is a novel inhibitor.Keywords
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