Cloning and expression of a novel rat GABAA receptor
- 27 March 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 246 (1-2) , 145-148
- https://doi.org/10.1016/0014-5793(89)80271-6
Abstract
Two full‐length cDNA clones encoding α‐ and β‐subunits of a GABAA receptor have been isolated from a rat cerebral cortex cDNA library. The mature α‐subunit protein consists of 428 amino acids with a calculated M r of 48680. This protein is highly homologous (∼99% amino acid identity) with the bovine brain α1‐subunit receptor [(1988) Nature 335, 76–79]. The mature rat β‐subunit receptor is a 448 amino acid polypeptide and shares ∼ 80% amino acid identity with the previously characterized bovine GABAA receptor β‐subunit [(1987) Nature 328, 221–227]. Co‐expression of the cloned DNA in Xenopus oocytes produces a functional receptor and ion channel with pharmacological characteristics of a GABAA receptor. GABAA α‐ and β‐subunit mRNA is detectable in the cortex, cerebellum and hippocampus.Keywords
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