STUDIES ON THE REACTION BETWEEN THE BLOOD-GROUP ANTIBODY ANTI-D AND ERYTHROCYTES

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Abstract
The reaction between 3 examples of the human blood-group antibody anti-D and red cells was investigated by using 131I-labeled antibody. The range of the equilibrium constants, K, extended from 1.4x108 to 7-2x10101.mole-1. The minimum range of the rate constant for association of 1 example of anti-D antibody was 3.0x104-6.3x1041.mole-1sec.-1. The minimum observed ranges of the rate constant for dissociation for each of the 3 examples of anti-D were 3.4x10-4-6.1x105 , 1.5x10-4-1.0x10-5 and 3-6x10-5-1.1x10-6sec.-1. The following thermodynamic constants were obtained for 1 example of antibody: standard enthalpy change, [DELTA]H , 0[plus or minus] 0.7 kcal./mole; standard entropy change, [DELTA]So, 41[plus or minus]2 cal./degree/mole. The number of D antigen sites on each red cell of the probable genotype R1R1 was estimated to be approximately 24,000.