Assimilation of liquid hydrocarbon by microorganisms. II. Growth kinetics

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Abstract
The growth kinetics of a microorganism with high affinity for liquid hydrocarbon which has a low solubility in water was investigated for Candida intermedia IFO 0761 in our previous work.6 The microorganism contained a hydrocarbon pool in and/or on the cell. The transfer of water‐soluble substrates to the cell was not the rate‐limiting step in the growth of C. intermedia accompanied by clump formation with liquid hydrocarbon. The operating conditions necessary for the oxygen supply for the growth were adequate for the growth of C. intermedia on n‐tetradecane. The saturation kinetics was valid for the specific growth rate of C. intermedia and specific concentration of hydrocarbon per unit cell mass; the specific growth rate was expressed by the following equation: