Glutathione S-transferase isoenzymes in human renal carcinoma demonstrated by immunohistochemistry

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Abstract
Glutathione S-transferase (GST) in man comprise at least four gene families. Three of these families give rise to cytosolic isoenzymes (α, μ and π classes), whilst the remainder is membrane bound and has been called microsomal GST. These enzymes are implicated in tumorogenesis and both π class GST and α class GST have been described in four cases of human renal cell carcinoma. Using specific polydonal rabbit antisera we have demonstrated by immunohisto-chemistry that all 12 renal carcinomas studied contained GST π. Most tumours also contained GST α, GST μ and microsomal GST isoenzymes but their distribution was heterogeneoud and sometimes very focal. This heterogeneity of GST isoenzyme distribution within tumours has not been well documented previously, but is relevant to our understanding of the functions of GST, and to the interpretation of biochemical quantification experiments using tissue extracts.

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