Amino acid sequence surrounding the lipoic acid cofactor of bovine kidney 2‐oxoglutarate dehydrogenase complex
- 28 September 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 222 (1) , 211-214
- https://doi.org/10.1016/0014-5793(87)80221-1
Abstract
The 2-oxoglutarate dehydrogenase complex was succinylated using 2-oxo[5-14C]glutarate in the presence of N-ethylmaleimide to label the lipoic acid cofactor of the transuccinylase (E2) component. Following peptic digestion, 14C-lipoate-containing peptides were purified and subjected to automated Edman degradation and amino acid analysis. The amino acid sequence surrounding the lipoyllysine residue is reported.Keywords
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