A novel nucleosome assembly procedure (with a little help from pectin)
- 22 February 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 318 (1) , 27-29
- https://doi.org/10.1016/0014-5793(93)81320-y
Abstract
The acidic polysaccharide pectin (α‐1,4‐polygalacturonic acid) has been introduced as a nucleosome assembly facilitator as a substitute for polyglutamic acid. The pectin‐assembled nucleosomes were indistinguishable from polyglutamic acid‐assembled nucleosomes by thermal denaturation and DNAse I digestion. Pectin had two major advantages over polyglutamic acid ‐ the yield of assembled cores was approximately 50% higher and the pectin could be easily removed after completion of the reassembly procedure by dialysis following pectinase cleavage.Keywords
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