Heavy metal resistantArthrobacter sp. — A tool for studying conjugational plasmid transfer between Gram-negative and Gram-positive bacteria

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Abstract
The role of two heavy metal‐resistant strains of the Gram‐positive genus Arthrobacter sp. as a tool in studying conjugational plasmid transfer between Gram‐positive and Gram‐negative bacteria is described. The high nickel resistance and the cobalt resistance of Arthrobacter sp. strain RM1/6 could be transferred to Arthrobacter sp. strain WS14. IncQ plasmids (pKT240, pKT240::czc, pML10) could be mobilized from E. coli into Arthrobacter spp. strains; antibiotic (Km, Ap, Tc)1) and heavy metal (Co) resistance genes were expressed in the recipient strains. IncQ plasmid pKT240 could be mobilized between Arthrobacter spp. strains. IncP plasmid RP4::Tn4371 was transferred from A. eutrophus to Arthrobacter sp., RP4‐mediated antibiotic resistance to Km was expressed in the recipient strain.