Contributions of ubiquitin- and PCNA-binding domains to the activity of Polymerase in Saccharomyces cerevisiae

Open Access

23 January 2007

journal article
research article
Published by Oxford University Press (OUP) in Nucleic Acids Research

Vol. 35 (3) , 881-889
https://doi.org/10.1093/nar/gkl1102

Abstract

Bypassing of DNA lesions by damage-tolerant DNA polymerases depends on the interaction of these enzymes with the monoubiquitylated form of the replicative clamp protein, PCNA. We have analyzed the contributions of ubiquitin and PCNA binding to damage bypass and damage-induced mutagenesis in Polymerase η (encoded by RAD30 ) from the budding yeast Saccharomyces cerevisiae . We report here that a ubiquitin-binding domain provides enhanced affinity for the ubiquitylated form of PCNA and is essential for in vivo function of the polymerase, but only in conjunction with a basal affinity for the unmodified clamp, mediated by a conserved PCNA interaction motif. We show that enhancement of the interaction and function in damage tolerance does not depend on the ubiquitin attachment site within PCNA. Like its mammalian homolog, budding yeast Polymerase η itself is ubiquitylated in a manner dependent on its ubiquitin-binding domain.

Keywords

UBIQUITIN

This publication has 45 references indexed in Scilit:

Replication of damaged DNA in mammalian cells: new solutions to an old problem
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 2002
RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO
Nature, 2002
Lesion bypass in yeast cells: Pol eta participates in a multi-DNA polymerase process
The EMBO Journal, 2002
Interaction with PCNA Is Essential for Yeast DNA Polymerase η Function
Molecular Cell, 2001
The Y-Family of DNA Polymerases
Published by Elsevier ,2001
The puzzle of PCNA's many partners
BioEssays, 2000
Two RING finger proteins mediate cooperation between ubiquitin-conjugating enzymes in DNA repair
The EMBO Journal, 2000
Mechanisms of accurate translesion synthesis by human DNA polymerase eta
The EMBO Journal, 2000
Low fidelity DNA synthesis by human DNA polymerase-η
Nature, 2000
Fidelity and Processivity of Saccharomyces cerevisiae DNA Polymerase η
Journal of Biological Chemistry, 1999