Isozyme Variation Among California Almond Cultivars: I. Inheritance

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Abstract
Horizontal starch gel electrophoresis was used to study the inheritance of some isozyme variants in almond seedling and almond-peach hybrid populations. In almond, aspartate aminotransferase (AAT), glucose phosphate isomerase (GPI), and leucine aminopeptidase (LAP) have two zones of activity, one of which is monomorphic in each case. The polymorphic loci, AAT-1 and GPI-2 each had two alleles and behaved as dimers in the electrophoretic analysis. LAP-1 behaved like a monomeric enzyme with two functional alleles, in addition to an hypothesized null allele. PGM behaved like a monomeric enzyme specified by two polymorphic loci, Pgm-1 and Pgm-2. Pgm-1 displayed two alleles, while Pgm-2 apparently had three, although one gave aberrant segregation ratios. Acid phosphatase (AcP), glyceraldehyde-phosphatase dehydrogenase (GAP), and 6-phosphogluconate dehydrogenase (6-PGD) isozymes showed two zones of activity in the almond cultivars assayed and produced no allelic variation. In peach, AAT-1 and GPI-2 had the same Rf values as almond and segregated in typical Mendelian ratios in F1 and F2 almond × peach populations. The alleles for LAP-1 and LAP-2 not only had different mobilities in almond and peach but were tightly linked to each other within each species. Thus, four-banded patterns were produced in the F1, which segregated in the F2 to peach, almond, and hybrid phenotypes with a reduced number of recombinants.

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