Circulating Reticulocytes in Normal Adults as Determined by the New Methylene Blue Method

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Abstract
Reticulocyte counts of 48 normal men and 30 normal women were determined by the new methylene-blue method. The mean value for men was 1.65% (95% limits = 0.8 to 2.5%); for women, 2.45% (95% limits = 0.8 to 4.1%). The difference between the reticulocyte counts of men and women is highly significant. New methylene blue, like brilliant cresyl blue, stains siderotic granules in siderocytes. Therefore, in the presence of large numbers of circulating siderocytes, such as may occur following splenectomy, the reticulocyte count by either method may be erroneous. Under these circumstances, reticulocytes are best enumerated on preparations which have been first stained for reticulum and then counterstained with the Prussian blue stain.

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