BIOCHEMICAL BASIS OF NITROBLUE TETRAZOLIUM REDUCTION IN NORMAL HUMAN AND CHRONIC GRANULOMATOUS DISEASE POLYMORPHONUCLEAR LEUKOCYTES

  • 1 January 1976
    • journal article
    • research article
    • Vol. 48  (2) , 309-313
Abstract
Normal human polymorphonuclear leukocytes (PMN) placed in anaerobic chambers reaching PO2''s [O2 tension] of less than 5 mm Hg fail to generate O2-, iodinate ingested particles and stimulated glucose-1-14C oxidation through the hexose monophosphate shunt. The observation that anaerobic cells were incapable of generating O2- or reducing nitroblue tetrazolium (NBT) to formazan supported the idea that NBT reduction in phagocytizing PMN was due exclusively to O2-dependent O2- generating oxidase which was deficient in chronic granulomatous disease leukocytes, despite their hyperphagocytic capacity.

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