Establishment of multiple lymphoblastoid lines from single lymph nodes and study of their immunoglobulin synthesis

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Abstract
Biopsies of one normal lymph node and two lymphomas were explanted into a number of independent cultures. Lymphoblastoid lines were established from a high proportion of the cultures from each biopsy. The immunoglobulins synthesized by each line were characterized and used as cell markers in an attempt to obtain information on the cellular origin of the lines. The majority of the lines produced, at the beginning of their culture history, several types of heavy and light chains, but this pattern evolved in the course of cultivation towards the production of only one species of immunoglobulin. Lines from the same lymph node differed from each other by the types of immunoglobulins produced, both initially and at a later stage. The results suggest that lymphoblastoid lines are derived from a non‐clonal population of precursor cells, and argue against the neoplastic nature of these cells.