The application of perpendicular and forward light scatter to assess nuclear and cellular morphology
- 1 September 1984
- Vol. 5 (5) , 515-522
- https://doi.org/10.1002/cyto.990050513
Abstract
Perpendicular and forward light scatter have been employed in a multiparametric approach to distinguish the various lines of the transplantable Dunning R3327 rat prostatic adenocarcinoma. Perpendicular light scatter correlated with nuclear size and shape. The average intensity of forward light scatter and perpendicular light scatter signals was demonstrated to increase as cells became less well-differentiated. The combination of perpendicular and forward light scatter allows for the discrimination of histologically indistinguishable tumors and may therefore be useful to establish a system of grading cancer.This publication has 10 references indexed in Scilit:
- Elastic and inelastic light scattering in flow cytometryCytometry, 1983
- A New Method to Assess Metastatic Potential of Human Prostate Cancer: Relative Nuclear RoundnessJournal of Urology, 1982
- Relationship Between Grade and Stage of adenocarcinoma of the Prostate and Regional Pelvic Lymph Node MetastasesJournal of Urology, 1982
- GENETIC INSTABILITY COUPLED TO CLONAL SELECTION AS A MECHANISM FOR TUMOR PROGRESSION IN THE DUNNING R-3327 RAT PROSTATIC ADENOCARCINOMA SYSTEM1982
- Histologic Grading of Primary Prostatic Cancer: A New Approach to an Old ProblemJournal of Urology, 1980
- Chromomycin A3 as a fluorescent probe for flow cytometry of human gynecologic samples.Journal of Histochemistry & Cytochemistry, 1977
- Pulse-height light-scatter distributions using flow-systems instrumentation.Journal of Histochemistry & Cytochemistry, 1976
- ANALYSIS OF CELL POPULATIONS WITH A FLUORESCENCE‐ACTIVATED CELL SORTER*Annals of the New York Academy of Sciences, 1975
- Differential Light Scattering from Spherical Mammalian CellsBiophysical Journal, 1974
- The Small-Angle Light Scattering of Biological CellsBiophysical Journal, 1970