Isolation and Chromatographic Analysis of Unidentified Fluorescence in Biological Fluids of Patients with Chronic Renal Disease

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Abstract
An unidentified fluorescent substance has recently been shown to increase in serum of patients with chronic renal failure. Further studies were made to gain more information as to its identity and to determine if it is present in other biological fluids. Molecular-exclusion chromatography and thin-layer chromatography provide evidence that the substance is present in dialysis fluids and ultrafiltrates of patients with chronic renal disease as well as in urine of normal individuals. Normal urine levels were 10-fold higher than the levels in serum of patients with chronic renal disease, and these serum levels were 50- to 110-fold higher than the levels in normal serum. The unknown substance is highly water soluble and has an emission maximum of 415 ± 5 mm and a relative molecular mass of less than 1,000. The described combination of gel chromatography and thin-layer chromatography is suggested as a useful approach for its isolation and subsequent identification.

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