Cloning and expression of cDNA for human vascular anticoagulant, a Ca2+‐dependent phospholipid‐binding protein
Open Access
- 1 July 1988
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 174 (4) , 585-592
- https://doi.org/10.1111/j.1432-1033.1988.tb14139.x
Abstract
Based on sequence information from tryptic peptides an almost full-size cDNA coding for the human vascular anticoagulant was isolated from a placental cDNA library and sequenced. The coding region was cloned into an Escherichia coli expression vector and the protein expressed at high levels. The recombinant protein was purified and found to be indistinguishable from its natural counterpart in several biological assays.This publication has 41 references indexed in Scilit:
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