Transcription initiation sites within an IS2insertion in a Gal-constitutive mutant ofEscherichia coli
- 1 January 1982
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (16) , 5015-5031
- https://doi.org/10.1093/nar/10.16.5015
Abstract
Insertion of the insertion sequence Is2(I) directly before the galE gene of the galactose operon results in a Gal minus phenotype (1, 2). The Gal-constitutive allele galc200 (and its deletion derivative galc200 delta 31) arise from such a Gal minus mutant by the insertion of LS2(II) DNA within the LS2(I) sequence (3). We have transcribed in vitro a DNA template representing the IS2-galE region of galc200 delta 31. Gal-directed transcription initiates at two sites within the IS2(I) sequence, 51 and 52 bp from the IS2-galE junction. The promoter for these transcripts, Pgal200 delta 31, is composed of a novel joint between a -10 region from the IS2(I) DNA and a -35 region contributed by the IS2(II) insertion. No promoters intrinsic to the 121 bp of the IS2(II) sequence also present on the template were detected. The relevance of Pgal200 delta 31 to the Galc phenotype of galc200 and to general mechanisms for the constitutive expression of genes adjacent to IS2 is discussed.Keywords
This publication has 31 references indexed in Scilit:
- Dual control for transcription of the galactose operon by cyclic AMP and its receptor protein at two interspersed promotersCell, 1977
- The 5′ terminal nucleotide of RNA from vesicular stomatitis virus defective interfering particlesVirology, 1977
- Insertion sequence IS2 near the gene for prophage λ excisionMolecular Genetics and Genomics, 1976
- Reversion of the gal3 mutation of Escherichia coli: Partial deletion of the insertion sequenceMolecular Genetics and Genomics, 1975
- IS2, a genetic element for turn-off and turn-on of gene activity in E. coliMolecular Genetics and Genomics, 1974
- Multiple copies of the insertion-DNA sequences IS1 and IS2 in the chromosome of E. coli K-12.1973
- Effect of Rho on Transcription of Bacterial OperonsNature New Biology, 1973
- Polar mutations in lac, gal and phage λ consist of a few IS-DNA sequences inserted with either orientationMolecular Genetics and Genomics, 1972
- Two kinds of insertions in bacterial genesMolecular Genetics and Genomics, 1972
- Nucleotide sequence of a ribonucleic acid transcribed in vitro from lambda phage deoxyribonucleic acid.1971